growth factor reduced basal membrane extract  (Trevigen)

Journal: PLoS ONE

Article Title: Cell Population Kinetics of Collagen Scaffolds in Ex Vivo Oral Wound Repair

doi: 10.1371/journal.pone.0112680

Figure Lengend Snippet: The ex vivo wound healing model is shown in top view and cross-sectional view (A). The bottom of the well was covered with an acellular growth factor reduced basal membrane extract (BME). The scaffold (sponge type scaffold composed of cross-linked collagen or the gel type scaffold composed of rat collagen I) in the center was surrounded by a BME-gel containing 800,000 hGFs/ml and covered with an acellular BME gel and Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 1% fetal bovine serum (FBS) and antibiotics. Both scaffold and gel were tested with and without the addition of platelet-derived growth factor-BB (PDGF). Fluorescence images were taken to assess DiI-labeled cells within the scaffold over time. To assess the metabolic activity and gene expression dynamics, the scaffolds including the “active zone” (dashed line) were subjected to MTT tests, gene array analysis, and quantitative PCR. (B) Representative scanning electron microscopy images depict the morphology of the collagen gel, collagen scaffold at 400×, and 800× magnification. The white bar represents 100 µm.

Article Snippet: 48 well plates were first layered with 50 μL of 12 mg/ml growth factor reduced basal membrane extract (BME; CULTREX, TREVIGEN, Gaithersburg, MD, USA) without cells.

Techniques: Ex Vivo, Membrane, Modification, Derivative Assay, Fluorescence, Labeling, Activity Assay, Gene Expression, Real-time Polymerase Chain Reaction, Electron Microscopy